Abstract: In order to investigate the human-induced vibration performance of large-span composite floors, crowd excitation was simulated by the random method based on a normal footfall force curve and other individual factors such as crowd distribution and step frequency. Human-induced vibration responses of a large-span composite floor were analyzed with the help of the multi-support excitation and the time history analysis method, and multiple tuned mass dampers with different parameters were designed to control the vibration responses of the large-span floor. The actual dynamic properties of the composite floor under two distinct construction phases were tested, and vertical vibration responses of the composite floor with and without multiple tuned mass dampers under different crowd-induced excitations were measured. The research results show that the types and walking routes of human-induced excitations have significant effects on the floor responses and the large-span composite floor has very closely-spaced modes of vibration. The vertical acceleration responses induced by crowd excitation are relatively great under certain condition, and the human-induced responses of a structure with multiple tuned mass dampers decrease dramatically to meet the requirement of body serviceability.