赤子爱胜蚓碱性磷酸酶的分离纯化

The Purification of Alkaline Phosphatase fromEisenia Foetide Sarigng

摘要: 以赤子爱胜蚓(Eisenia Foetida Sarigng)整体为材料,采用常规碱性磷酸(酯)酶(AKP)纯化方法,即正丁醇抽提,硫酸铵分级沉淀,DEAE-纤维素柱层析,Sephadex G-100及Sephadex G-200凝胶过滤等,分离纯化了蚓体中的一种AKP,经PAGE鉴定达到电泳纯,比活性为40.64 U/mg蛋白,纯化倍数为57.24倍。
- 碱性磷酸酶 /
- 提纯 /
- 赤子爱胜蚓
Abstract: Alkaline phosphatase (AKP) was purified from the cell extracts ofEisenia Foetida Sarigngusing the routine means: n-bulanol extraction, ammonium sulfate fractional precipitation, DEAE-cellulose chromatography, Sephadex G-100 and Sephadex G-200 gel filtration. The purified AKP was found to be homogeneous by polyacrylamide gel disc electrophoresis. Its specific activity is 40.64 units per milligram of protein, and purification multiple is 57.24-fold.
- alkaline phosphatase /
- purification /
- Eisenia Foetide Sarigng

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