Investigation into Screening,Identifying and Solid-State Fermentation of Cellulolytic Strains
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摘要: 从腐烂稻草、土壤和牛粪等样品中分离到12株能在以CMC-Na(羧甲基纤维素钠)为唯一碳源的固体培养基上生长的菌株,并从中筛选出1株分解纤维素能力较强的真菌,初步鉴定为脉纹孢菌(Neurospora sp.).通过对其固态发酵条件进行单因素优化试验,初步确定了发酵培养基最佳碳、氮源种类及添加量:稻草粉与麸皮的质量比为9;1,NH4NO3 0.5%;最适培养条件为:固液比1;3,接种量1 mL,pH值自然,培养温度30℃,培养时间96 h.该菌株的羧甲基纤维素酶活和滤纸酶活分别达到3 551.1和386.7μmol/(g.h).
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关键词:
- 脉纹孢菌(Neurosporasp.) /
- 纤维素酶 /
- 固态发酵
Abstract: Twelve strains were isolated from putrid straw,wood,soil and cattle feces etc. They can grow in a solid medium with(sodium carboxymethyl cellulose) CMC-Na as an only carbon source.One strain of fungi,preliminarily identified as Neurospora sp.,with a strong ability to decompose cellulose was screened from the 12 strains by a series of experiments.The experiment of single factor optimization about the solid-state fermentation of this strain was carried out.The research results shows that to the strain,the optimal carbon and nitrogen sources are that the mass ratio of straw to bran is(9 to 1,) and NH4NO3 0.5%;and that the optimal conditions for culture are that the inoculum’s size is 1 mL,the ratio of solid to liquid 1 to 3,culture temperature around 30 ℃ and cultivating time 96 h under the condition of a natural pH value.The carboxyl methyl cellulase activity and the filter paper activity of Neurospora sp.are 3551.1 and 386.7 μmol/(g·h) respectively under these optimal zymotic conditions.-
Key words:
- Neurospora sp. /
- cellulase /
- solid-state fermentation
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ROSANNE M.Measurement of saccharification by cellulase[J].Enzyme and Microbial Technology,1985 (7):586-587.[2] 迟乃玉,张庆芳,刘长江,等.纤维素酶高产菌株最适发酵条件的研究[J].沈阳农业大学学报,2000,31(4):380-382.[3] WILKE C R.Preliminary cost analysis for enzymatic hydrolysis of newsprint[J].Biotechnology Bioengineering Symposium,1976(6):155-176.[4] 郑佐兴,段明星,徐文联,等.高活性纤维素酶菌株的筛选及其产酶条件的研究[J].微生物学杂志,1996,16(1):35-38.[5] 魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979:445-446.[6] 蒋传葵,金承德,吴仁龙,等.工具酶的活力测定[M].上海:上海科学出版社,1982:79-81.[7] 邬敏辰,李江华.里氏木霉固体发酵生产纤维素的研究[J].江苏食品与发酵,1998(2):2-6.[8] 李如亮.生物化学实验[M].武汉:武汉大学出版社,1996:9-10.[9] CAI J M.Production of xylanase by aspergillus niger A3 using solid-state fermentation[J].Industrial Microbiology,1997,27(3):1-4.[10] 沈萍.微生物学[M].北京:高等教育出版社,2000:426-427.[11] 冯炘,王丹,辛丽霞,等.粗糙脉孢菌(Neurospora crassa)产纤维素酶发酵条件研究[J].食品科学,2005:26(1):67-70.FENG Xin,WANG Dan,XIN Lixia,et al.Study on fermentation conditions of cellulase produced by neurospora crassa[J].Food Science,2005,26(1):67-70. -
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