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高效产纤维素酶曲霉生物转化纤维素乙醇

刘洋 邱忠平 孟涛 龚正君 王东梅 樊超

刘洋, 邱忠平, 孟涛, 龚正君, 王东梅, 樊超. 高效产纤维素酶曲霉生物转化纤维素乙醇[J]. 西南交通大学学报, 2020, 55(1): 225-230. doi: 10.3969/j.issn.0258-2724.20180477
引用本文: 刘洋, 邱忠平, 孟涛, 龚正君, 王东梅, 樊超. 高效产纤维素酶曲霉生物转化纤维素乙醇[J]. 西南交通大学学报, 2020, 55(1): 225-230. doi: 10.3969/j.issn.0258-2724.20180477
LIU Yang, QIU Zhongping, MENG Tao, GONG Zhengjun, WANG Dongmei, FAN Chao. Biotransformation of Bioethanol from Lignocellulose by High Yield Cellulase-Producing Aspergillus[J]. Journal of Southwest Jiaotong University, 2020, 55(1): 225-230. doi: 10.3969/j.issn.0258-2724.20180477
Citation: LIU Yang, QIU Zhongping, MENG Tao, GONG Zhengjun, WANG Dongmei, FAN Chao. Biotransformation of Bioethanol from Lignocellulose by High Yield Cellulase-Producing Aspergillus[J]. Journal of Southwest Jiaotong University, 2020, 55(1): 225-230. doi: 10.3969/j.issn.0258-2724.20180477

高效产纤维素酶曲霉生物转化纤维素乙醇

doi: 10.3969/j.issn.0258-2724.20180477
基金项目: 国家自然科学基金(21776230),四川省科技支撑计划(2015NZ0097)
详细信息
    作者简介:

    刘洋(1978—)女,讲师,博士,研究方向为环境微生物,E-mail:liuyang2010@home.swjtu.edu.cn

    通讯作者:

    邱忠平(1967—),女,副教授,博士,研究方向为固体废弃物处理与资源化,E-mail:zhpqiu@sina.com

Biotransformation of Bioethanol from Lignocellulose by High Yield Cellulase-Producing Aspergillus

  • 摘要: 为了提高纤维素乙醇的生产效率,对高效产纤维素酶曲霉W-10的产酶条件进行优化,利用其发酵所得的粗酶液对预处理后的水稻秸秆粉进行酶解,并用酿酒酵母通过同步糖化发酵(simultaneous saccharification and fermentation, SSF)工艺生物转化纤维素乙醇. 首先通过定时取样测定还原糖量,研究不同底物浓度、不同表面活性剂添加量、纤维二糖酶的协同作用等因素对酶解过程的影响. 然后利用所得优化后的酶解条件进行同步糖化实验,研究不同的发酵温度、发酵时间、初始pH值等影响因素对同步糖化发酵乙醇的影响. 结果表明,当底物浓度为80 g/L、表面活性剂吐温-80添加浓度为5 g/L、酶解体系外纤维二糖酶补加量为166.67 nkat/g时,粗酶液的酶解率最高;在35 ℃的培养温度、初始pH值为5的条件下发酵4 d时,发酵液中乙醇含量最高,乙醇得率可达0.43 g/g(底物干重). 优化高效产纤维素酶曲霉W-10酶解水稻秸秆的反应条件,可促进纤维素乙醇生物转化技术的发展,有利于可再生的清洁能源生物乙醇的商业化生产和应用.

     

  • 图 1  不同反应条件对酶解反应的影响

    Figure 1.  Effects of different reaction conditions on hydrolyse

    图 2  不同反应条件对SSF的影响

    Figure 2.  Effects on SSF from different reaction conditions

    表  1  水稻秸秆的纤维素、半纤维素、木质素质量分数

    Table  1.   Mass fraction of cellulose,hemicellulose and lignin in rice stalk %

    纤维素半纤维素木质素
    42.4829.4010.73
    下载: 导出CSV
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出版历程
  • 收稿日期:  2018-06-01
  • 修回日期:  2018-08-14
  • 网络出版日期:  2019-01-11
  • 刊出日期:  2020-02-01

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