基于微球孔内团聚的多孔钛表面药物释放技术

王东微; 肖东琴; 段可; 鲁雄; 冯波; 翁杰

doi:10.3969/j.issn.0258-2724.2015.04.020

基于微球孔内团聚的多孔钛表面药物释放技术

doi: 10.3969/j.issn.0258-2724.2015.04.020

王东微^1,2,3,
肖东琴^1,2,3,
段可^1,2, ,,
鲁雄^1,2,
冯波^1,2,
翁杰^1,2

基金项目:

国家973计划资助项目(2012CB933600)

国家自然科学基金资助项目(81071456, 51172188)

教育部博士点基金资助项目(20100184120011)

四川省科技创新苗子工程计划资助项目(2012ZZ065)

详细信息

作者简介:
王东微(1985-),女,博士研究生,研究方向为生物材料表面改性,E-mail:wdw_swjtu@163.com

通讯作者:
段可(1973-),男,副教授,研究方向为材料表面技术,E-mail:keduan2@gmail.com

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出版历程
- 收稿日期: 2013-10-24
- 刊出日期: 2015-08-25

Microsphere Agglomeration Technique for Drug Release from Porous-Coated Ti

WANG Dongwei^1,2,3,
XIAO Dongqin^1,2,3,
DUAN Ke^{1,2
, ,},
LU Xiong^1,2,
FENG Bo^1,2,
WENG Jie^1,2

摘要

摘要: 通过含药物的微球在骨植入材料表面微孔内团聚方法解决现有骨植入材料表面药物释放技术缺乏通用性、无法控制释放速度的问题.用乳液法合成负载不同含量庆大霉素的壳聚糖微球粉末,并使其铺散于多孔钛层表面后进入孔内.经水润湿后,微球在孔内团聚并被固定于孔内.实验结果表明:水浴振荡试验7 d(100 r/min, 37 ℃)后, 80%~83%的微球仍位于多孔钛层内,微球-多孔钛复合物表现出初期(24 h)快速释放和后期( 24 h)缓慢释放;金黄色葡萄球菌培养48 h后,多孔钛与载庆大霉素微球的复合物产生了抑菌圈.
- 多孔钛 /
- 微球 /
- 团聚 /
- 骨植入材料 /
- 药物释放
Abstract: A microsphere agglomeration technique incorporating gentamicin in porous-coated Ti was used to overcome technical disadvantages associated with available drug-release techniques for bone implants. Chitosan microspheres containing various concentrations of gentamicin were prepared by a water-in-oil emulsion method and lyophilized to dry powders. The powders were then spread on porous-coated Ti, and wetted with water to promote their agglomeration after they enter into the pores by gently tapping the samples. Microscopical analyses revealed that, the microspheres formed submilllimeter-sized agglomerates and entrapped in the pores. After immersion in shaking water bath (100 r/min, 37 ℃) for 7 d, 80%-83% of the agglomerates were retained in the coating, suggesting a reasonable entrapment stability. The microsphere-porous Ti complexes showed a rapid drug release in 24 h followed by a prolonged slow release. After coculture with Staphylococcus aureus for 48 h, the porous Ti carrying gentamicin-loaded microspheres produced clear inhibition zones, confirming its In vitro antibacterial activity.
- porous Ti /
- microsphere /
- agglomeration /
- bone implant /
- drug release

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参考文献(48)

KAZEMZADEH-NARBAT M, KINDRACHUK J, DUAN K, et al. Antimicrobial peptides on calcium phosphate-coated titanium for the prevention of implant-associated infections

[J]. Biomaterials, 2010, 31(36): 9519-9526.

ROSENBERG A G. Cementless acetabular components: the gold standard for socket revision

刘宣勇. 硬组织植入材料表/界面研究进展

[J]. Journal of Arthroplasty, 2003, 18(3): 118-120.

DUAN K, WANG R. Surface modification of bone implants through wet chemistry

STIGTER M, BEZEMER J, DE GROOT K, et al. Incorporation of different antibiotics into carbonated hydroxyapatite coatings on titanium implants, release and antibiotic efficacy

[J]. 无机材料学报,2011,26(1): 1-11. LIU Xuanyong. Progress in research on the surface/interface of materials for hard tissue implant

STROBEL C, BORMANN N, KADOW-ROMACKER A, et al. Sequential release kinetics of two (gentamicin and BMP-2) or three (gentamicin, IGF-I and BMP-2) substances from a one-component polymeric coating on implants

HOLWARTH J M, MA P X. Biomimetic nanofibrous scaffolds for bone tissue engineering

[J]. Journal of Inorganic Materials, 2011, 26(1): 1-11.

RADIN S, CAMPBELL J T, DUCHEYNE P, et al. Calcium phosphate ceramic coatings as carriers of vancomycin

[J]. Journal of Materials Chemistry, 2006, 16(24): 2309-2321.

PULEO D A, NANCI A. Understanding and controlling the bone-implant interface

张静哲,李东东,刘桂锋. 新型介孔羟基磷灰石/壳聚糖-万古霉素药物释放系统复合材料的制备及体外抗菌和成骨能力

PENG W, GRAINGER D W. Drug/device combinations for local drug therapies and infection prophylaxis

[J]. Journal of Controlled Release, 2004, 99(1): 127-137.

VARDE N K, PACK D W. Microspheres for controlled release drug delivery

[J]. Journal of Controlled Release, 2011, 156(1): 37-45.

NIU X, FENG Q, WANG M, et al. Porous nano-HA/collagen/PLLA scaffold containing chitosan microspheres for controlled delivery of synthetic peptide derived from BMP-2

LI J, LI S, VAN BLITTERSWIJK C A, et al. A novel porous Ti6Al4V: characterization and cell attachment

[J]. Biomaterials, 2011, 32(36), 9622-9629.

NIU X, FENG Q, WANG M, et al. Preparation and characterization of chitosan microspheres for controlled release of synthetic oligopeptide derived from BMP-2

国家环境保护局. GB/T 118931989 水质总磷的测定:钼酸铵分光光度法

HANZLIK J A, DAY J S. Bone ingrowth in well-fixed retrieved porous tantalum implants

[J]. Biomaterials, 1997, 18(11): 777-782.

JI J, HAO S, WU D, et al. Preparation, characterization and in vitro release of chitosan nanoparticles loaded with gentamicin and salicylic acid

[J]. Biomaterials, 1999, 20(23): 2311-2321.

HETRICK E M, SCHOENFISCH M H. Reducing implant-related infections: active release strategires

BELT H, NEUT D, SCHENK W, et al. Infection of orthopedic implants and the use of antibiotic-loaded bone cements: a review

[J]. 高等学校化学学报,2012,33(2): 219-224. ZHANG Jingzhe, LI Dongdong, LIU Guifeng. In vitro evaluation of antibacterial and osteogenic properties and synthesis of mesoporous hydroxyapatite/chitosan composite loaded with vancomycin as a drug delivery

MARTINO A D, SITTINGER M, RISBUD M V. Chitosan: a versatile biopolymer for orthopaedic tissue-engineering

任俊,沈健,卢寿慈. 颗粒分散科学与技术

[J]. Chemical Journal of Chinese Universities, 2012, 33(2): 219-224.

MALYNYCH S, LUZINOV I, CHUMANOV G. Poly (vinyl pyridine) as a universal surface modifier for immobilization of nanoparticles

[J]. Biomaterials, 2006, 27(11): 2450-2467.

[J]. Expert Opinion on Biological Therapy, 2004, 4(1): 35-51.

[J]. Journal of Controlled Release, 2009, 134(2): 111-117.

[J]. Journal of Biomedical Materials Research Part A, 2005, 73(2): 223-233.

[J]. Journal of Microencapsulation, 2009, 26(4): 297-305.

[S]. 北京:中国标准出版社,1989.

[J]. Journal of Arthroplasty, 2013, 28(6): 922-927.

[J]. Carbohydrate Polymers, 2011, 85(4): 803-808.

[J]. Chemical Society Reviews, 2006, 35(9): 780-789.

[J]. Acta Orthopaedica, 2001, 72(6): 557-571.

[J]. Biomaterials, 2005, 26(30): 5983-5990.

[M]. 北京: 化学工业出版社,2005: 102-103.

[J]. Journal of Physical Chemistry B, 2002, 106(6): 1280-1285.

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基于微球孔内团聚的多孔钛表面药物释放技术

doi: 10.3969/j.issn.0258-2724.2015.04.020

作者简介: 王东微(1985-),女,博士研究生,研究方向为生物材料表面改性,E-mail:wdw_swjtu@163.com

通讯作者: 段可(1973-),男,副教授,研究方向为材料表面技术,E-mail:keduan2@gmail.com

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Microsphere Agglomeration Technique for Drug Release from Porous-Coated Ti

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目录

作者简介:
王东微(1985-),女,博士研究生,研究方向为生物材料表面改性,E-mail:wdw_swjtu@163.com

通讯作者:
段可(1973-),男,副教授,研究方向为材料表面技术,E-mail:keduan2@gmail.com