高温退火氧化钛粉末的血液相容性实验研究

赵安莎; 王昭; 周烁; 朱晓华; 谢骁; 黄楠

doi:10.3969/j.issn.0258-2724.2014.02.027

高温退火氧化钛粉末的血液相容性实验研究

doi: 10.3969/j.issn.0258-2724.2014.02.027

赵安莎¹,
王昭¹,
周烁¹,
朱晓华¹,
谢骁²,
黄楠^1, ,

基金项目:

国家973计划资助项目（9732011CB606200）

中央高校基本科研业务费专项资金资助项目（SWJTU11CX054）

详细信息

作者简介:
赵安莎（1977-），女，副教授，硕士生导师，研究方向为生物材料表面改性、生物传感器表面设计加工及应用等，E-mail：anshazhao@263.net

通讯作者:
黄楠（1956-），男，教授，博士生导师，研究方向为生物材料表面处理等，E-mail：nhuang@263.net

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出版历程
- 收稿日期: 2013-06-05
- 刊出日期: 2014-03-25

Blood Compatibility of Titanium Oxide Powder after High Temperature Annealing

摘要

摘要: 利用真空高温退火技术制备不同退火温度的Ti-O粉末、X线衍射仪、Zeta电位和比表面积等表征材料，采用Bradford法测纤维蛋白原在其表面的吸附解吸附行为，运用红外线光谱分析纤维蛋白原在其表面吸附后二级结构的变化，用流式细胞仪研究其对血小板的激活程度.研究表明，800 ℃高温退火使得Ti-O粉末由锐钛矿转化为金红石，比表面积减少10倍，携带有更多的负电荷；吸附血浆纤维蛋白原的数量减少到67 g，且维持原有的二级结构，并存在较大的解吸附；不易引起血小板的激活，具有更好的血液相容性.
- 氧化钛 /
- 退火 /
- 蛋白质 /
- 血液相容性 /
- 界面
Abstract: Vacuum annealing was used to prepare the Ti-O powder at different annealing temperatures. The structure and surface properties were characterized by X-ray diffraction, Zeta potential test and surface area measurement respectively. Bradford method was applied to determine the adsorption and desorption of fibrinogen on the powder, and Fourier transform infrared spectroscopy was used to analyze the change of secondary structure of adsorbed fibrinogen on the samples. Then flow cytometry was used to measure the degree of platelets activation. The results show that after 800 ℃ annealing process, the crystal structure of Ti-O powder transformed from anatase to rutile with 10 times less total surface area and more negative charges, and the amount of fibrinogen adsorption was decreased to 67g and retained its normal conformation. Less platelet was activated by the annealed Ti-O powder, which proves that it has an excellent blood-compatibility.
- titanium oxide /
- annealing /
- protein /
- blood compatibility /
- interface

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参考文献(16)

RAFFAINI G, GANAZZOLI F. Protein adsorption on biomaterial and nanomaterial surfaces: a molecular modeling approach to study non-covalent interac-tions[J]. Journal of Applied Biomaterials and Biomechanics, 2010, 8(3):135-45.

RABE M, VERDES D, SEEGER S. Understanding protein adsorption phenomena at solid surfaces[J]. Advance Colloid Interface Science, 2011, 162(1/2): 87-106.

CACCIAFESTA P, HUMPHRIS A D L, JANDT K D, et al. Human plasma fibrinogen adsorption on ultraflat titanium oxide surfaces studied with atomic force microscopy[J]. Langmuir, 2000, 16(21): 8167-8175.

程青, 王国斌. 生物材料血液相容性评价研究进展[J]. 上海生物医学工程, 2001, 2(3): 31-33. CHENG Qing, WANG Guobing. Progress in the blood compatibility research of biomaterials[J]. Shanghai Journal of Biomedical Engineering, 2001, 2(3): 31-33.

周烁. 氧化钛材料与血浆关键蛋白质的界面反应行为及与凝血的关系. 成都:西南交通大学, 2012.

DIEBOLD U. The surface science of titanium dioxide[J]. Surface Science Reports, 2003, 48(5-8): 53-299.

梁薇. 微孔材料BET比表面积计算中相对压力应用范围研究[J]. 工业催化, 2006, 14(11): 67-69. LIANG Wei. Calculation of BET surface area of microporous materials at different relative pressue[J]. Industrial Catalysis, 2006, 14(11): 67-69.

王孝平, 刑树礼. 考马斯亮蓝法测定蛋白含量的研究[J]. 天津化工, 2009, 23(3): 40-41. WANG Xiaoping, XING Shuli. Braford method for the determination of protein content[J]. Tianjin Chemical Industry, 2009, 23(3): 40-41.

Mac DONALD D E, DEO N, MARKOVIC B. Adsorption and dissolution behavior of human plasma fibronectinon thermallyand chemicallymodified titanium dioxide particles[J]. Biomaterials, 2002, 23(4): 1269-1279.

DASGUPTA S, BANDYOPADHYAY A, BOSE S. Reverse micelle-mediated synthesis of calcium phosphate nanocarriers for controlled release of bovine serum albumin[J]. Acta Biomater, 2009, 5(8): 3112-3121.

NORDE W. Adsorption of proteins from solution at the solid-liquid interface[J]. Colloid Interface Science, 1986, 25(4): 267-340.

BENNETT J S. Platelet-fibrinogen interactions[J]. Annals of the New York Academy of Sciences, 2001, 936(6): 340-354.

TUNC S, MAITZ M F, STEINER G, et al. In situ conformational analysis of fibrinogen adsorbed on Si surfaces[J]. Colloids and Surfaces B: Biointerfaces, 2005, 42(25): 219-225.

JUNG S Y, LIM S M, ALBERTORIO F, et al. The Vroman effect: a molecular level description of fibrinogen displacement[J]. Journal of the American Chemical Society, 2003, 125(42): 12782-12786.

MATSUMOTO T, OKAZAKI M, INOUE M, et al. Hydroxyapatite particles as a controlled release carrier of protein[J]. Biomaterials, 2004, 25(17): 3807-3812.

BLOMBCK B, HESSEL B, HOGG D, et al. A two-step fibrinogen-fibrin transition in blood coagulation[J]. Nature, 1978, 275(5680): 501-505.

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