Abstract: A vascular bioreactor was designed and constructed by Beihang University and Sichuan University for Southwest Jiaotong University for simulating in vivo environments. The performance indicators of the bioreactor including shearing stress, the state of laminar flow, and pulse pressure were analyzed quantitatively through theoretical derivation of hydromechanics and orthogonal experiment range analysis. The results show that for blood vessels of different diameters, the bioreactor could provide shearing stress ranging from 0.08 to 70.51 Pa. In order to maintain a laminar flow condition of the perfusate, the maximum flow rate in the tubes of five kinds of diameters should not exceed 4.36 cm3/s when the inlet tube length of the culture chamber was 18 cm, and the viscosity coefficient and fluid density of the perfusate were fixed. The operating distance of the pulsation generator had a bigger effect on the difference between systolic and diastolic blood pressures than the flow and frequency, while the effect of pressure was the smallest.