Abstract: In order to test the rationality of the air-conditioning system design of high-speed trains, an entire geometry model for compartment and air-conditioning system of high-speed trains was built considering the characteristics of negative pressure at fresh-air inlet, complicated air-conditioning pipes and good airtightness. The airflow field in the entire geometry was simulated by solving the governing differential functions of computational fluid dynamics (CFD) using the finite volume method. The distributions of velocity field, temperature field, humidity field, and CO2 concentration in the chamber were obtained, and the thermal comfort was evaluated by flow field indexes and comfort indexes. The results show that when a train is running at 350 km/h in summer, the temperature distribution is uniform in compartments; the average value of CO2 concentration is 0.07% to meet the demand for comfort (less than 0.15%); because of the high wind velocity, effective temperature difference at aisle is -4.5 ℃, which is 2.8 ℃ below the standard value of the thermal comfort index; and an appropriate air supply and recycle mode is crucial to obtain uniform flow field and good thermal comfort.