Selection and Fermentation of Mixed Cellulolytic Strains
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摘要: 为了提高纤维素的降解能力,对降解纤维素的混合菌的筛选和发酵条件进行了试验研究.从土壤样品中分离、筛选出一组对纤维素具有高效降解作用的混合菌,初步鉴定为毛霉菌(Mucor sp.)和曲霉菌(Aspergillus sp.).通过试验确定了该组混合菌发酵产酶的最佳条件:稻草粉与麸皮的质量比为8:1,2.5%的硫酸铵为补充氮源,固液比为1:1.5,pH值自然,种龄为3 d的菌丝接种,培养温度为28℃,培养时间为5 d.当接种量为20%,接种比例为2:1时,羧甲基纤维素酶活最大为7 324.1μmol/(g.h);当接种量为30%,接种比例为1:1时,滤纸酶活最大为753.4μmol/(g.h).Abstract: A kind of mixed strains to be able to decompose straw cellulose efficiently was screened from soil and identified as Aspergillus sp.and Mucor sp.The solid-state fermentation of the mixed strains was optimized by orthogonal single factor experiments.The experimental results show that the optimal solid-state cultivation conditions are as follows: inoculating mycelium cultivated for 3 d,a straw-bran mass ratio of 8 to 1,(NH4)2SO4 with a content of 2.5% as supplement nitrogen resource,a solid-liquid mass ratio of 1 to 1.5,a natural pH value,cultivation temperature of around 28℃ and cultivation time of 5 d.When inoculation gross is 20% and inoculation ratio of Mucor sp.to Aspergillus sp.is 2 to 1,7 324.1 μmol/(g·h)as the highest carboxyl methyl cellulase(CMC) activity can be achieved,but when the inoculation gross and the inoculation ratio are 30% and 1 to 1 respectively,753.4 μmol/(g·h) as the highest filter paper activity(FPA) can be acquired.
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Key words:
- cellulose /
- mixed strain /
- solid-state fermentation /
- orthogonal experiment
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