Research on Syntheses of PU Microcapsule for Gene Engineering Cell
-
摘要: 采用预聚-扩链-中和-分散溶解法,一步合成聚氨酯水溶液,再用凝聚相分离法合成聚氨酯微胶囊.探讨了聚氨酯水溶液和聚氨酯微胶囊的合成条件,包括二异氰酸酯单体种类对微胶囊的影响,聚乙二醇分子量对聚氨酯微胶囊制备的影响,总单体含量对聚氨酯微胶囊形态的影响,甲苯二异氰酸酯与聚乙二醇配比的影响及反应时间、反应温度和搅拌速度等的影响.用红外光谱、差示扫描量热仪和扫描电子显微镜对聚氨酯微胶囊进行表征,测出聚氨酯微胶囊直径2 mm,内腔直径50~500μm,膜孔直径20~100 nm.研究表明,合成稳定的膜孔径一定且具有微相分离结构的聚氨酯微胶囊的最佳条件是:预聚温度为60℃,扩链温度为50℃,中和温度为30℃;预聚和扩链时间均为2 h;预聚和扩链搅拌速度为500~600 r/min,分散溶解的搅拌速度为700~800 r/min.
-
关键词:
- 聚氨酯微胶囊 /
- 预聚-扩链-中和-分散溶解 /
- 凝聚相分离法 /
- 微相分离结构
Abstract: Polyurethane(PU) water solutions were synthesized by combining the prepolymerization,chain-extension,neutralization and dispersion-diffluence methods in one step.And PU microcapsules were synthesized with the coacervation phase separation method.The synthetic conditions of PU water solutions and PU microcapsules were investigated,including the effects of the kinds of diisocyanate,the feed ratios of toluene-2,4-diisocyanate(TDI) and polyethylene glycol(PEG),reaction temperature,reaction time and stirred velocity on the morphology of PU microcapsules.The PU microcapsules were synthesized with TDI,PEG,dimethylol propionic acid(DMPA) and triethylamine(TEA).The structure and morphology of the PU microcapsules were measured with infrared spectrum(IR),differential scanning calorimetry(DSC) and scanning electron microscope(SEM).The diameter of the globose PU microcapsules is 2 mm or so,the cavity diameter in the PU microcapsules is 50 to 500 μm,and the membrane orifice diameter of the PU microcapsules is 20 to 100 nm.Furtheremore,the optimal conditions of synthesizing the stable, good morphology and microphase separation structural PU microcapsules were given.These conditions are as follows: the prepolymerization,chain-extension and neutralization temperatures are 60,50 and 30℃ respectively,both the times of prepolymerization and chain-extension are 2 h,the stirring velocities of prepolymerization and chain-extension are 500 to 600 r/min,and the stirring velocity of dissolution is 700 to 800 r/min. -
宋健,陈磊,李效军.微胶囊化技术及应用[M].北京:化学工业出版社,2001:20-60.[2] GIOVANNI L,GIUSEPPE B,RICCARDO C,et al.Multifunctional microcapsules for pancreatic islet cell entrapment:design,preparation and in vitro characterization[J].Biomaterials,2003,24:3 101-3 114.[3] 肖秀斌,张伟京,薛毅珑,等.APA-BCCs微胶囊移植对晚期癌症患者的镇痛效果评价[J].中国临床康复,2004,8(20):3 953-3 955.XIAO Xiubin,ZHANG Weijing,XUE Lilong,et al.Clinical evaluation of analgesic effect of APA-BCCs xenotransplantation in patients with advanced cancer[J].Chinese Journal of Clinical Rehabilitation,2004,8 (20):3 953-3 955.[4] Chang T M S.Semipermeable microcapsules[J].Science,1964,146:524-525.[5] 李洁华,谢兴益,何成生,等.医用聚氨酯生物相容性研究新进展[J].生物医学工程学杂志,2002,19(2):315-319LI Jiehua,XIE Xingyi,HE Chengsheng,et al.The advanced of research for biocompatibility of medical polyurethanes[J].Journal of Biomedical Engineering,2002,19 (2):315-319.[6] MOON H T,LEE Y K,HAN J K,et al.A nevol formulation for controlled release of heparin-DOCA conjugate dispersed as nanoparticles in polyurethane film[J].Biomaterials,2001,22(2):281-289.[7] JABBARI E,KHAKPOUR M.Morphology of and release behavior from porous polyurethane microspheres[J].Biomaterials,2000,21(6):2 073-2 079.[8] HONG K,PARK S.Characterization of ovalbumin-cintaining polyurethane microcapsules with different structures[J].Polymer Testing,2000,19(5):975-984. -
点击查看大图
计量
- 文章访问数: 1307
- HTML全文浏览量: 77
- PDF下载量: 384
- 被引次数: 0
下载: