Experimental Study on Micronucleus in Root-Tip Cells of Vicia faba Induced by Three Pesticides
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摘要: 用蚕豆根尖微核技术研究3种农药(甲胺磷、甲基硫菌灵和盐酸吗啉胍)在正常使用浓度范围的诱变效应.结果表明,3种农药单独使用时,诱发蚕豆根尖细胞微核率与对照组相比均有不同程度的上升(p0.01),农药浓度与微核率呈剂量-效应关系,且盐酸吗啉胍的诱变效应大于其他2种农药.3种农药联合作用时,高浓度组具拮抗性,中低浓度组显示协同作用,微核率与农药浓度不表现剂量-效应关系.3种农药的最佳使用浓度:甲胺磷为0.230 0~0.2500 g·L-1,甲基硫菌灵为0.4680~0.5260 g·L-1,盐酸吗啉胍为0.0556~0.0592 g·L-1.Abstract: Micronucleus(MCN) test of Vicia faba root-tip was conducted to study the mutation inducing effect of three pesticides: methamidophos(Me),thiophanate-methyl(Th) and moroxydine hydrochloride(Mo) in their normally used concentrations.When each of the three pesticides is used alone,the micronucleus frequencies(MNF) of Vicia faba root-tips of the three test groups are higher than those of the control group(P0.01),showing a dosage effect,and Mo gives more obvious inductive effect than the other two pesticides do.When the three pesticides are used together,the high concentration groups show antagonistic actions,the middle and the low concentration groups show synergistic actions,and different concentrations of the pesticides do not exhibit dosage effects.The optimum concentrations of the pesticides are 0.230 0 to 0.250 0 g·L-1 for Me,0.468 0 to(0.526 0 g·L-1)for Th,and 0.055 6~0.059 2 g·L-1for Mo.
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