Abstract: To resolve the real-time problem in data acquisition, a new real-time data acquisition method was proposed. In this method, actively sending data to serialport is created on intelligent equipment and a thread program of serialport monitoring is created based on multithreading technology. Handshake and end-connecting processes in conventional serialport communication are taken out from the procedure of data acquisition, and the reliability of the system can be guaranteed. The experimental results show that data transmitting time is shortened to 20% of the original transmitting time when transmitted data are 20 bytes per time. The proposed method has been applied to practice.