• ISSN 0258-2724
  • CN 51-1277/U
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Volume 21 Issue 4
Aug.  2008
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Article Contents
ZHANG Jianqiang, WANG Yankun, LI Yong. Selection and Fermentation of Mixed Cellulolytic Strains[J]. Journal of Southwest Jiaotong University, 2008, 21(4): 549-554.
Citation: ZHANG Jianqiang, WANG Yankun, LI Yong. Selection and Fermentation of Mixed Cellulolytic Strains[J]. Journal of Southwest Jiaotong University, 2008, 21(4): 549-554.

Selection and Fermentation of Mixed Cellulolytic Strains

  • Received Date: 25 Feb 2008
  • Publish Date: 25 Aug 2008
  • A kind of mixed strains to be able to decompose straw cellulose efficiently was screened from soil and identified as Aspergillus sp.and Mucor sp.The solid-state fermentation of the mixed strains was optimized by orthogonal single factor experiments.The experimental results show that the optimal solid-state cultivation conditions are as follows: inoculating mycelium cultivated for 3 d,a straw-bran mass ratio of 8 to 1,(NH4)2SO4 with a content of 2.5% as supplement nitrogen resource,a solid-liquid mass ratio of 1 to 1.5,a natural pH value,cultivation temperature of around 28℃ and cultivation time of 5 d.When inoculation gross is 20% and inoculation ratio of Mucor sp.to Aspergillus sp.is 2 to 1,7 324.1 μmol/(g·h)as the highest carboxyl methyl cellulase(CMC) activity can be achieved,but when the inoculation gross and the inoculation ratio are 30% and 1 to 1 respectively,753.4 μmol/(g·h) as the highest filter paper activity(FPA) can be acquired.

     

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