Callus Induction and PlantRegeneration from Bulbs of Fritillaria thunbergiiM iq.

The effects ofmediums, phytohormones and phytohormone combinations on induction of calluses and protocorm-like bodies (PLBs) from the explants of bulbs ofFritillaria thunbergiiMiq.. The induction ofcalluses and PLBs is closely related tomedium, hormone combination and the type of the explants. The best callus induction is achieved inMurashige-Skoog (MS) medium supplemented with 1. 0 mg/L 2, 4-D (2, 4-dichlorophenoxyacetic acid)+1. 5 mg/L NAA (1-naphthalene acetic acid)+300 mg/L casein hydrolysate+0. 55% (mass proportion) agar by taking the bulbs of Fritillaria thunbergiiMiq. as explants. Plantlets are bestregenerated from snuff-colored calluses of the bulbs ofFritillaria thunbergiiMiq. inMSmedium supplementedwith 1. 0 mg/LNAA+2. 0 mg/L ZT (zeatin)+0. 55% casein hydrolysate. The bestmedium forPLBs induction isMS supplemented with 1. 0 mg/L 2, 4-D+2. 0 mg/LKT (6-furfurylaminopurine)+0. 55% casein hydrolysate.