In order to investigate the structural behavior of perfobond rib shear connectors in the whole load-bearing process, 37 specimens of perfobond rib shear connectors, sorted into 11 groups, were tested in the failure test under static loading. The load-slip curve in each working region and failure mode was studied, the influences of the shear stiffness of concrete bars and the tension bearing capacity of perforated rebar on the load-slip relationship were analyzed using the normalization method, and a uniform calculation formula of load-slip relationship was established. The results show that the load-slip relationship in elastic region is linear, whose slope is mainly affected by the shear stiffness of the concrete bars. In elastoplastic and hardening regions, the load-slip relationship is a power law, whose coefficient is linear with the shear stiffness of concrete bars and the tension bearing capacity of steel bars. The rational diameter of the steel bars in the perfobond rib shear connector with concrete bars of diameter 60 and 45 mm, is 20 and 16 mm, respectively.